Submit Manuscript  

Article Details


Optimization of Microwave-assisted Extraction of Bioactive Compounds from Dunaliella bardawil Using RSM and ANFIS Modeling and Assessment of the Anticancer Activity of Bioactive Compounds

[ Vol. 5 , Issue. 2 ]

Author(s):

Selvaraj Kunjiappan*, Theivendren Panneerselvam, Suthendran Kannan, Balasubramanian Somasundaram, Murugesan Sankaranarayanan, Sankarganesh Arunachalam and Arockiasamy Manimaran   Pages 139 - 154 ( 16 )

Abstract:


Background: Algae gain is of much importance in food and pharmaceutical industries due to the presence of rich bioactive compounds.

Methods: A Microwave Assisted Extraction (MAE) was engaged to enhance recovery of bioactive compounds from Dunaliella bardawil. The effective experimental condition was identified by optimization using Response Surface Methodology (RSM) coupled with Central Composite Rotatable Design (CCRD) along with Adaptive Neuro-Fuzzy Inference System (ANFIS). In this connection, four independent parameters, namely, n-hexane concentration (X1:60-85%), microwave power (X2:15- 25%), radiation-induced temperature (X3:40-60°C) and radiation exposure time (X4:7.5-12.5min) were chosen.

Results: The optimum conditions resulted in 72.34-75.86% of biomass (y1) and 46.27-47.17 mg of β- carotene equivalents/g to biomass (y2). In addition, the corresponding antioxidant activities showed 85.65-85.90% in DPPHsc (y3), 81.30-81.31% in ABTSsc (y4) and 59.65-62.45 µgmol (Fe (II))/g in FRAP (y5). Further, the bioactive compounds, β-carotene, and lutein were identified through HPLC and the biomass exhibited significant anticancer activity against oral epithelial cancer cells.

Conclusion: The observed results clearly highlight that the study has great scope and value for the continuation of further exploitation and application.

Keywords:

ANFIS, anticancer, antioxidant, Dunaliella bardawil, RSM, bioactive compounds.

Affiliation:

Sir CV Raman-KS Krishnan International Research Centre, Kalasalingam Academy of Research and Education, Krishnankoil- 626126, Tamilnadu, Department of Pharmaceutical Chemistry, Karavali College of Pharmacy, Near Reymond, Vamanjoor, Mangaluru-575028, National Cyber Defence Research Centre, Kalasalingam University, Krishnankoil-626126, Virudhunagar, Sir CV Raman-KS Krishnan International Research Centre, Kalasalingam Academy of Research and Education, Krishnankoil- 626126, Tamilnadu, Department of Pharmacy, Birla Institute of Technology and Science, Pilani- 333031, Rajasthan, Department of Biotechnology, School of Bio and Chemical Engineering, Kalasalingam Academy of Research and Education, Krishnankoil- 626126, Tamilnadu, PG and Advanced Research, Department of Advanced Zoology and Biotechnology, Government Arts and Science College, Chennai-600035, Tamilnadu

Graphical Abstract:



Read Full-Text article